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springerlab
Flow-Cytometry-Toolkit
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Flow-Cytometry-Toolkit
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fcsparsewell.m
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master
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Flow-Cytometry-Toolkit
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fcsparsewell.m
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function [data, meta] = fcsparsewell(datadir, platename, wellid)
% FCSPARSEWELL loads fcs data from a file whose name begins with the string
% PLATENAME and whose header identifies it as being from well WELLID. The
% function relies on the metadata contained in the FCS header to determine
% the well corresponding to a certain file.
%
% This function used to be called LOADWELLDATA and FCSREADWELL but was
% renamed to be consistent with other functions.
%
% Example:
%
% fcsreadwell('../data/','plate1','A01')
%
% loads data for well A01 from a file such as 'plate1_001_001.fcs'
%
% Created 20130310, updated 20141021 by JW
% load filename cache
global wellfilenameindex;
try
load([datadir 'wellfilenameindex.mat']);
catch
disp('No filename cache found. Generating...');
makewellfilenameindex(datadir);
end
% standardize wellid string
if ischar(wellid)
[r,c] = well2coord(wellid);
wellid = coord2well(r,c);
elseif isnumeric(wellid) && numel(wellid) == 2
r = wellid(1);
c = wellid(2);
wellid = coord2well(r,c);
else
error('Please input [row, column] or a string like ''A01'' as the 3rd argument to FCSPARSEWELL.');
end
fieldname = genvarname(platename);
if isfield(wellfilenameindex,fieldname) && isfield(wellfilenameindex.(fieldname),wellid)
fn = wellfilenameindex.(fieldname).(wellid);
[data meta] = fcsparse([datadir fn],'rename');
else
warning(['Plate/well not found or not in cache: ' platename ' ' wellid]);
data=[];
meta=[];
end
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